Binary concepts and standardization in counter-current separation technology

Research output: Research - peer-reviewArticle

  • 4 Citations

Abstract

Counter-current separation (CS) technology is currently faced with the challenge of being fit for the purpose of omics analysis, which involves highly complex samples and digitized research environments. Resembling a network of binary decisions, CS requires standardization of operation parameters in order to be efficient. While recent CS engineering solutions uniformly involve centrifugal force designs to overcome the limitation of the earth's 1 × g force, factors of instrument design, operation, and graphical representation of the outcome are equally important targets for standardization. For example, chromatograms that emphasize the unique K-based nature of CS, such as reciprocal symmetry (ReS) plots, foster the fundamental understanding of CS operation. Because significant differences exist in underlying mechanism (e.g., stationary phase volume), outcome (e.g., construction of chromatograms), and scale (e.g., factors affecting overall method sensitivity) of solid-liquid vs. liquid-liquid chromatography technologies, standardization will enable the systematic exploration of the differential properties of the two LC technologies, and will be key to making CS fit for the digital omics age.

LanguageEnglish
Pages4237-4244
Number of pages8
JournalJournal of Chromatography A
Volume1216
Issue number19
DOIs
StatePublished - May 8 2009

Fingerprint

Standardization
Technology
Liquids
Liquid Chromatography
Research
Liquid chromatography
Earth (planet)

Keywords

  • Binary
  • Counter-current chromatography
  • Metabolomics
  • Partition
  • Reciprocal symmetry plots
  • Standardization

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Binary concepts and standardization in counter-current separation technology. / Friesen, J. B.; Pauli, G. F.

In: Journal of Chromatography A, Vol. 1216, No. 19, 08.05.2009, p. 4237-4244.

Research output: Research - peer-reviewArticle

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