The use of proteomics studies in identifying moonlighting proteins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Proteomics studies that characterize hundreds or thousands of proteins in parallel can play an important part in the identification of moonlighting proteins, proteins that perform two or more distinct and physiologically relevant biochemical or biophysical functions. Functional assays, including ligand-binding assays, can find a surprising second function for a protein that was previously identified as performing a different function, for example, a DNA-binding ability for an enzyme in amino acid metabolism. The results of large-scale assays of protein–protein interactions, gene knockouts, or subcellular protein localizations, or bioinformatics analysis of amino acid sequences and three-dimensional structures, can also be used to predict that a protein has additional functions, but in these cases it is important to use biochemical and biophysical methods to confirm the protein can perform each function.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages437-443
Number of pages7
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1871
ISSN (Print)1064-3745

Fingerprint

Proteomics
Proteins
Gene Knockout Techniques
Protein Sequence Analysis
Computational Biology
Ligands
Amino Acids
DNA
Enzymes

Keywords

  • Moonlighting proteins
  • Multifunctional proteins
  • Protein function prediction
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Jeffery, C. J. (2019). The use of proteomics studies in identifying moonlighting proteins. In Methods in Molecular Biology (pp. 437-443). (Methods in Molecular Biology; Vol. 1871). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8814-3_25

The use of proteomics studies in identifying moonlighting proteins. / Jeffery, Constance J.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 437-443 (Methods in Molecular Biology; Vol. 1871).

Research output: Chapter in Book/Report/Conference proceedingChapter

Jeffery, CJ 2019, The use of proteomics studies in identifying moonlighting proteins. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1871, Humana Press Inc., pp. 437-443. https://doi.org/10.1007/978-1-4939-8814-3_25
Jeffery CJ. The use of proteomics studies in identifying moonlighting proteins. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 437-443. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8814-3_25
Jeffery, Constance J. / The use of proteomics studies in identifying moonlighting proteins. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 437-443 (Methods in Molecular Biology).
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